The script averages the same as ffmpeg into one file only it does so with enfuse/hugin_align_image instead.
It is possible to use ffmpeg to create and use darkframes. Check page 50 here:
http://www.astro-electronic.de/FFmpeg_Book.pdf
Thanks for this clarification, Danne. I understand now. By the way, I love your two averaging presets - perfect for noise reduction in low-light photography. I am trying to figure out which of all these options provide the best results for such type of photography and are most user friendly too:
1) Dark frame subtraction using an Averaged MLV, as Masc suggests;
2) ISO averaging (requires a tripod);
3) Frame burst (requires a tripod);
4) Silent picture using Dual ISO (typically 100/800 and 200/1600);
5) Garry's hand held bracketing scrips.
6) Using "magic" ISO numbers to improve dynamic range (ISO research).
All these are remarkable ML options that can all be handled by MLVApp quite nicely. Personally, I have been using option #4 a lot when traveling since it allows handheld shots with a stabilized lens at shutter speeds down to 1/4 s. with a very low count of misfocused shots. Taking multiple handheld shots with the intervalometer to select the sharpest one further reduces the chance that you end up with a missed shot. ISO averaging seems a better option, though, since it allows working at closed apertures - better for wide-angle low-light landscape photography.
It would be nice if more of you share your experience on low-light photography using the above options.