I tried stacking the data today and got some strange results with the 50s and 49s darks combined together. Fortunately I also had a set of staggered 49 and 50s dark frames so I had almost and equal amount of darks to calibrate together and independently.

on my first try I calibrated everything together and scaled the darks because I used 50 and 49s.

light_BINNING_1_integration

Calculating noise standard deviation...

* Channel #0

σR = 9.257e-005, N = 1909520 (10.59%), J = 3

* Channel #1

σG = 8.183e-005, N = 321791 (1.79%), J = 4

* Channel #2

σB = 9.191e-005, N = 338895 (1.88%), J = 4

on the second try I applied calibration frames independently to 49s and 50s. After applying indepent calibration frames I stacked the 49 and 50s frames together and got much lower read noise, but it was a little higher in green?

integration

Calculating noise standard deviation...

* Channel #0

σR = 1.072e-004, N = 212831 (1.18%), J = 4

* Channel #1

σG = 8.856e-005, N = 365346 (2.03%), J = 4

* Channel #2

σB = 1.001e-004, N = 395618 (2.19%), J = 4

A1ex, I will try and figure out how to adjust the script to give consistent subs, thanks for the tip.