Focus stacking

[dinobike]

A macro shot of a needle and thread using focus stacking

[garry23]

Could you provide some info, eg: lens, aperture number of slices etc etc.

[dinobike]

Could you provide some info, eg: lens, aperture number of slices etc etc.

Of course!

Camera: Canon 700D
Lens: Canon 100mm F2.8 + 3 tube extensions(36mm+20mm+12mm)+ 1.4X extender
Aperture: f/14
T: 0,3 sec.
Iso: 6400
Slices: 19
Light: Natural
Post: Lightroom and Photoshop

[Walter Schulz]

This will give you about 3:1 magnification and therefore an effective aperture of about
(m + 1) * f = (3 + 1) * 14 = 56
If you go pixelpeeping you will find some amount of softness caused by diffraction. If you want to use your shots in larger format and want to preserve detail you might want to use smaller aperture numbers.
Not a problem with smaller representations like the one shown above.

[dinobike]

This will give you about 3:1 magnification and therefore an effective aperture of about
(m + 1) * f = (3 + 1) * 14 = 56
If you go pixelpeeping you will find some amount of softness caused by diffraction. If you want to use your shots in larger format and want to preserve detail you might want to use smaller aperture numbers.
Not a problem with smaller representations like the one shown above.

Thank you walter!, i notice that too (diffraction) but i didn't understand it until your explanation
Im shooting now with an aperture of  f3.5.
Here's my latest try:

[rpt]

The Canna plant on the terrace flowered and I used the Focus Stacking feature in Magic Lantern that I have on my 5D3 and stacked 29 images to get this one. I used LR for the edit and PS to stack (blend).
F/8
1/200
ISO 100

Canna Focus stacked_1280 by Rustom Taraporevala, on Flickr